Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride
Identifieur interne : 005005 ( Main/Exploration ); précédent : 005004; suivant : 005006Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride
Auteurs : David Parkinson [Canada] ; James D. Redshaw [Canada]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1984.
English descriptors
Abstract
Several proteins, which are used as molecular weight markers in polyacrylamide gel electrophoresis, were reacted with dabsyl chloride. This labeled them deep orange and the chromophore attachment was stable throughout the electrophoretic procedure and fixation. Small amounts (10–50 μg) of the labeled proteins could be loaded onto gels and seen with the unaided eye so that the separation during electrophoresis could be followed. Dabsylation did not affect the mobility of the proteins. The location of the orange band gave a good indication of the position of the protein in the gel so that molecular weight estimations could be made during and immediately following electrophoresis.
Url:
DOI: 10.1016/0003-2697(84)90434-2
Affiliations:
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